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Dentin decontamination using chloramine T prior to experiments involving bacteria

Lookup NU author(s): Dr Sarah Rolland, Professor Angus Walls, Professor John McCabe

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Abstract

Objectives: This study aims to investigate the importance of ISO11405 recommended storage regime for extracted teeth in surface disinfectant chloramine T (chlT) prior to use in biofilm or in vitro caries studies involving microorganisms. ChlT may be absorbed into dentin and undergoes breakdown with organic material. Methods: Extracted roots were stored in chlT (2 days), rinsed and transferred to distilled deionised water. HPLC at regular intervals determined chlT elution. At 4 weeks roots were boiled in water and eluent assessed with HPLC. ChlT breakdown (┬▒organic material) over time was monitored with HPLC. ChlT minimum inhibitory/bactericidal concentration (MIC/MBC) against Lactobacillus acidophilus was evaluated using L. acidophilus broth and chlT serial dilutions. Results: No significant increase in chlT elution was detected between 2 h and 4 weeks (ANOVA, Tukeys, p > 0.05), although levels tended to increase with time. ChlT detected in water was 0.005%, corresponding to 0.05% in dentin. After boiling (4 weeks) chlT breakdown products in water corresponded to 0.015% in dentin. MIC/MBC of chlT against L. acidophilus was 0.031%. Significance: ChlT breakdown is accelerated by organic material. L. acidophilus is highly sensitive to chlT. ChlT readily leaches from dentin but rinsing does not reduce chlT concentration below MIC/MBC. Low levels of chlT may remain but will probably be in a less active form. Teeth disinfected in chlT for use in research involving bacteria must be stored in distilled water for at least 2 h to reduce chlT concentration below MBC, although longer will give greater elution and breakdown. ┬ę 2007 Academy of Dental Materials.


Publication metadata

Author(s): Rolland SL, Carrick TE, Walls AW, McCabe JF

Publication type: Article

Publication status: Published

Journal: Dental Materials

Year: 2007

Volume: 23

Issue: 12

Pages: 1468-1472

Print publication date: 01/12/2007

ISSN (print): 0109-5641

ISSN (electronic): 1879-0097

Publisher: Elsevier Inc.

URL: http://dx.doi.org/10.1016/j.dental.2007.01.001

DOI: 10.1016/j.dental.2007.01.001

PubMed id: 17349688


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