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In Childhood Acute Lymphoblastic Leukemia, Blasts at Different Stages of Immunophenotypic Maturation Have Stem Cell Properties

Lookup NU author(s): Dr Simon BomkenORCiD, Kerrie Wilson, Professor Julie Irving, Professor Hermann Josef Vormoor

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Abstract

We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL. © 2008 Elsevier Inc. All rights reserved.

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Author(s): le Viseur C, Hotfilder M, Bomken S, Wilson K, Röttgers S, Schrauder A, Rosemann A, Irving JAE, Stam R, Shultz L, Harbott J, Jürgens H, Schrappe M, Pieters R, Vormoor HJ

Publication type: Article

Publication status: Published

Journal: Cancer Cell

Year: 2008

Volume: 14

Issue: 1

Pages: 47-58

Print publication date: 08/07/2008

ISSN (print): 1535-6108

ISSN (electronic): 1878-3686

Publisher: Cell Press

URL: http://dx.doi.org/10.1016/j.ccr.2008.05.015

DOI: 10.1016/j.ccr.2008.05.015

PubMed id: 18598943

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