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Expression of oestrogen and progesterone receptors in gastric cancer: a flow cytometric study

Lookup NU author(s): Dr Daya Karat, Dr Ian Brotherick, Dr Brian Shenton, Dr Debbie Scott, Professor Michael Griffin

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Abstract

increased expression of oestrogen (ER) and progesterone (PR) receptors have been reported in gastric adenocarcinoma, although results have been variable. Immunohistochemical staining methodologies, in particular in the detection of ER, have been inconsistent with many tumours being classified ER-negative. In this study we have used flow cytometry to quantify expression of ER and PR in gastric adenocarcinoma and examine their relationships with established prognostic indicators. Cytokeratin-positive cells obtained from tumour biopsies of 50 patients with gastric cancer and ten control patients were labelled with biotinylated ER or PR antibodies followed by streptavidin PE. Flow cytometry was seen to increase the detection of ER levels in gastric cancer with more receptor-positive patients in this study than in results published to date. We believe this is related to the sensitivity of the flow cytometric assay with the detection of small shifts in ER level detected using cytokeratin gating. On analysis, the data showed no significant correlations with tumour stage and grade, and no differences were seen between normal mucosa and gastric cancer samples.

Publication metadata

Author(s): Karat D, Brotherick I, Shenton BK, Scott D, Raimes SA, Griffin SM

Publication type: Article

Publication status: Published

Journal: British Journal of Cancer

Year: 1999

Volume: 80

Issue: 8

Pages: 1271-1274

Print publication date: 01/06/1999

ISSN (print): 0007-0920

ISSN (electronic): 1532-1827

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/sj.bjc.6690497

DOI: 10.1038/sj.bjc.6690497

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