Browse by author
Lookup NU author(s): Mohammed Mukhtar, Dr Mark Stubbs, Professor Loranne Agius
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Glucokinase is rapidly exported from the nucleus of hepatocytes in response to a rise in glucose or fructose l-P, We demonstrate using confocal microscopy and quantitative imaging that in contrast to previous findings, the regulatory protein of glucokinase (GKRP) also translocates from the nucleus during substrate-induced translocation of glucokinase, However, the fractional decrease in nuclear GKRP is smaller than for glucokinase and is determined by the metabolic state and not by the distribution of glucokinase, Translocation of glucokinase and GKRP is not inhibited by leptomycin B, an inhibitor of exportin-1 function, These findings highlight the importance of quantitative imaging for determining nuclear export of proteins and suggest that GKRP may have a role in nuclear export or import of glucokinase. (C) 1999 Federation of European Biochemical Societies.
Author(s): Stubbs M; Agius L; Mukhtar M
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Year: 1999
Volume: 462
Issue: 3
Pages: 453-458
Print publication date: 03/12/1999
Online publication date: 30/11/1999
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: Elsevier
URL: https://doi.org/10.1016/S0014-5793(99)01580-X
DOI: 10.1016/S0014-5793(99)01580-X
Altmetrics provided by Altmetric