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Evidence for glucose and sorbitol-induced nuclear export of glucokinase regulatory protein in hepatocytes

Lookup NU author(s): Mohammed Mukhtar, Dr Mark Stubbs, Professor Loranne Agius


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Glucokinase is rapidly exported from the nucleus of hepatocytes in response to a rise in glucose or fructose l-P, We demonstrate using confocal microscopy and quantitative imaging that in contrast to previous findings, the regulatory protein of glucokinase (GKRP) also translocates from the nucleus during substrate-induced translocation of glucokinase, However, the fractional decrease in nuclear GKRP is smaller than for glucokinase and is determined by the metabolic state and not by the distribution of glucokinase, Translocation of glucokinase and GKRP is not inhibited by leptomycin B, an inhibitor of exportin-1 function, These findings highlight the importance of quantitative imaging for determining nuclear export of proteins and suggest that GKRP may have a role in nuclear export or import of glucokinase. (C) 1999 Federation of European Biochemical Societies.

Publication metadata

Author(s): Stubbs M; Agius L; Mukhtar M

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 1999

Volume: 462

Issue: 3

Pages: 453-458

Print publication date: 03/12/1999

Online publication date: 30/11/1999

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier


DOI: 10.1016/S0014-5793(99)01580-X


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