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Differential gene regulation by 9-cis and all-trans retinoic acid in neuroblastoma cells

Lookup NU author(s): Professor Penny Lovat, Dr Mark Dobson, Professor Archibald Malcolm, Professor Andrew Pearson, Dr Chris RedfernORCiD


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Background. 9-cis retinoic acid (RA) is more effective than all-trans RA at inducing neuroblastoma differentiation in vitro, and has distinct biological properties with respect to its ability to promote apoptosis in N-type neuroblastoma cells. The cellular effects of 9-cis RA may, in part, result from activation of retinoid X receptor (RXR) homodimers. If this hypothesis is correct, 9-cis RA may control the expression of a different subset of retinoid-regulated genes compared to all-trans RA. Procedure. We have therefore used differential mRNA display to identify genes differentially expressed in neuroblastoma cells in response to all-trans and 9-cis RA. Results. The majority of cDNAs differentially expressed in response to all-trans or 9-cis RA matched to nonredundant Genbank sequences or EST database sequences. Differential-display profiles were similar in SH SY 5Y and SH S EP cells, clonal derivatives of the mixed neuroblastoma cell line SK N SH, although there were apparent differences between these cell lines with respect to the retinoid-regulation of specific RT-PCR cDNA fragments. Conclusions. These data support the view that 9-cis and all-trans RA act via different receptor mechanisms. Med. Pediatr. Oncol. 36:135-138, 2001. (C) 2001 Wiley-Liss, Inc.

Publication metadata

Author(s): Lovat, P.E., Dobson, M., Malcolm, A.J., Pearson, A.D.J., Redfern, C.P.F.

Publication type: Article

Publication status: Unknown

Journal: Medical and Pediatric Oncology

Year: 2001

Volume: 36

Issue: 1

Pages: 135-138

ISSN (print): 1545-5009

ISSN (electronic): 1545-5017


DOI: 10.1002/1096-911X(20010101)36:1<135::AID-MPO1032>3.0.CO;2-4


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