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Perinuclear mRNA localisation by vimentin 3 '-untranslated region requires a 100 nucleotide sequence and intermediate filaments

Lookup NU author(s): Professor John Hesketh

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Abstract

The role of the vimentin 3 ' -untranslated region (3 ' -UTR) in mRNA localisation was studied in cells transfected with a reporter sequence linked to subregions of the 3 ' -UTR, Tn situ hybridisation showed that nucleotides 37-137, including a previously identified protein-binding domain, mere sufficient to localise transcripts to perinuclear cytoplasm. Transfection of two SW13 cell lines that do and do not express vimentin showed that perinuclear localisation due to either the vimentin or c-myc 3 ' -UTR requires intermediate filaments, The data suggest that both a specific protein-binding region of the vimentin 3 ' -UTR and intermediate filaments themselves are required to determine the site of vimentin synthesis. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Publication metadata

Author(s): Hesketh JE; Bermano G; Shepherd RK; Zehner ZE

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2001

Volume: 497

Issue: 2-3

Pages: 77-81

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/S0014-5793(01)02438-3

DOI: 10.1016/S0014-5793(01)02438-3

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