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Quantitative assessment of mixed chimerism in allogeneic stem cell transplant patients - A comparison of molecular genetic and cytogenetic approaches

Lookup NU author(s): Daniel Routledge, Dr David Bourn, Dr Nicholas Bown, Mike Cole, Professor Andrew Gennery, Dr Ann Curtis

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Abstract

After allogeneic stem cell transplantation, dual donor and recipient populations may be present. Donor/recipient ratio changes over time may predict clinical outcome: accurate measurement of these changes are needed. Chimerism may be measured by XY-fluorescence in situ hybridization for donor/recipient sex mismatch, or polymerase chain reaction amplification of short tandem repeat loci with donor/recipient sex match. Patients were monitored by each method. Additionally, mononuclear cells from 2 sex-mismatched individuals were mixed and analyzed using both methods. Each gave concordant estimates of patient chimerism and discriminated cell population ratios in mixed blood. We conclude that cytogenetic and molecular methods give accurate donor chimerism estimates.


Publication metadata

Author(s): Routledge DM, Jackson A, Bourn D, Bown NP, Cole M, Slatter MA, Gennery AR, Curtis A

Publication type: Article

Publication status: Published

Journal: Journal of Pediatric Hematology / Oncology

Year: 2007

Volume: 29

Issue: 6

Pages: 428-431

ISSN (print): 1077-4114

ISSN (electronic): 1536-3678

Publisher: Lippincott Williams & Wilkins

URL: http://dx.doi.org/10.1097/MPH.0b013e318064519a

DOI: 10.1097/MPH.0b013e318064519a

PubMed id: 17551410


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