Toggle Main Menu Toggle Search

Open Access padlockePrints

Purification of an alpha class glutathione S-transferase from melphalan-resistant Chinese hamster ovary cells and demonstration of its ability to catalyze melphalan-glutathione adduct formation

Lookup NU author(s): Dr Andrew Hall, Elizabeth Matheson, Dr Linda Hogarth


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


We have shown previously that a Chinese hamster ovary cell line (designated CHO-Chl(r)), generated by exposure to chlorambucil and demonstrating a greater than 20-fold collateral resistance to melphalan, showed increased expression of an a form of glutathione S-transferase (GST) associated with amplification of GST genes. Here, we demonstrate that GST purified from CHO-Ch(r) cells contains a form with a pI of 9, not present in CHO-K1 cells or Chinese hamster liver, which has the ability to accelerate the formation of glutathione-melphalan adducts. This result provides evidence that overexpression of the ct class GST may be directly responsible for the development of resistance to bifunctional alkylating agents.

Publication metadata

Author(s): Hall AG, Matheson E, Hickson ID, Foster SA, Hogarth LA

Publication type: Note

Publication status: Published

Journal: Cancer Research

Year: 1994

Volume: 54

Issue: 13

Pages: 3369-3372

Print publication date: 01/07/1994

ISSN (print): 0008-5472

ISSN (electronic): 1538-7445


PubMed id: 8012951