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Purification of an alpha class glutathione S-transferase from melphalan-resistant Chinese hamster ovary cells and demonstration of its ability to catalyze melphalan-glutathione adduct formation

Lookup NU author(s): Dr Andrew Hall, Elizabeth Matheson, Dr Linda Hogarth

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Abstract

We have shown previously that a Chinese hamster ovary cell line (designated CHO-Chl(r)), generated by exposure to chlorambucil and demonstrating a greater than 20-fold collateral resistance to melphalan, showed increased expression of an a form of glutathione S-transferase (GST) associated with amplification of GST genes. Here, we demonstrate that GST purified from CHO-Ch(r) cells contains a form with a pI of 9, not present in CHO-K1 cells or Chinese hamster liver, which has the ability to accelerate the formation of glutathione-melphalan adducts. This result provides evidence that overexpression of the ct class GST may be directly responsible for the development of resistance to bifunctional alkylating agents.

Publication metadata

Author(s): Hall AG, Matheson E, Hickson ID, Foster SA, Hogarth LA

Publication type: Note

Publication status: Published

Journal: Cancer Research

Year: 1994

Volume: 54

Issue: 13

Pages: 3369-3372

Print publication date: 01/07/1994

ISSN (print): 0008-5472

ISSN (electronic): 1538-7445

URL: http://cancerres.aacrjournals.org/cgi/reprint/54/13/3369

PubMed id: 8012951

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