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Lookup NU author(s): Dr Andrew Hall,
Dr Linda Hogarth
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We have shown previously that a Chinese hamster ovary cell line (designated CHO-Chl(r)), generated by exposure to chlorambucil and demonstrating a greater than 20-fold collateral resistance to melphalan, showed increased expression of an a form of glutathione S-transferase (GST) associated with amplification of GST genes. Here, we demonstrate that GST purified from CHO-Ch(r) cells contains a form with a pI of 9, not present in CHO-K1 cells or Chinese hamster liver, which has the ability to accelerate the formation of glutathione-melphalan adducts. This result provides evidence that overexpression of the ct class GST may be directly responsible for the development of resistance to bifunctional alkylating agents.
Author(s): Hall AG, Matheson E, Hickson ID, Foster SA, Hogarth LA
Publication type: Note
Publication status: Published
Journal: Cancer Research
Print publication date: 01/07/1994
ISSN (print): 0008-5472
ISSN (electronic): 1538-7445
PubMed id: 8012951