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Crystal structure of the dimer of two essential Salmonella typhimurium proteins, YgjD and YeaZ and calorimetric evidence for the formation of a ternary YgjD-YeaZ-YjeE complex

Lookup NU author(s): Dr Heather Lamb, Paul Thompson, Professor Alastair Hawkins

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Abstract

YgjD from COG0533 is amongst a small group of highly conserved proteins present in all three domains of life. Various roles and biochemical functions (including sialoprotease and endonuclease activities) have been ascribed to YgjD and orthologs, the most recent, however, is involvement in the post transcriptional modification of certain tRNAs by formation of N6-threonyl-adenosine (t6A) at position 37. In bacteria, YgjD is essential and along with YeaZ, YjeE, and YrdC has been shown to be ‘necessary and sufficient’ for the tRNA modification. To further define interactions and possible roles for some of this set of proteins we have undertaken structural and biochemical studies. We show that formation of the previously reported heterodimer of YgjD–YeaZ involves ordering of the C-terminal region of YeaZ which extends along the surface of YgjD in the crystal structure. ATPγS or AMP is observed in YgjD while no nucleotide is bound on YeaZ. ITC experiments reveal previously unreported binary and ternary complexes which can be nucleotide dependent. The stoichiometry of the YeaZ–YgjD complex is 1:1 with a KD of 0.3 µM. YgjD and YjeE interact only in the presence of ATP, while YjeE binds to YgjD-YeaZ in the presence of ATP or ADP with a KD of 6 µM. YgjD doesn't bind the precursors of t6A, threonine, and bicarbonate. These results show a more complex set of interactions than previously thought, which may have a regulatory role. The understanding gained should help in deriving inhibitors of these essential proteins that might have potential as antibacterial drugs.

Publication metadata

Author(s): Nichols CE, Lamb HK, Thompson P, Omari KE, Lockyer M, Charles I, Hawkins AR, Stammers DK

Publication type: Article

Publication status: Published

Journal: Protein Science

Year: 2013

Volume: 22

Issue: 5

Pages: 628-640

Print publication date: 01/05/2013

Online publication date: 26/03/2013

Acceptance date: 01/03/2013

ISSN (print): 0961-8368

ISSN (electronic): 1469-896X

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1002/pro.2247

DOI: 10.1002/pro.2247

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