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Lookup NU author(s): Dr Marie-Helene Ruchaud, Dr Katy Hester, Dr Jim Macfarlane, Professor John SimpsonORCiD, Professor Anthony De SoyzaORCiD
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BACKGROUND AND OBJECTIVE: Neutrophils are consistently found in inflamed and infected airways in idiopathic bronchiectasis but relatively little is known about the function of blood neutrophils in this condition. We hypothesised that peripheral blood neutrophil phagocytosis and superoxide generation is impaired in bronchiectasis and that granulocyte-macrophage colony-stimulating factor (GM-CSF) is capable of improving neutrophil function.METHODS: Neutrophils were isolated from the peripheral blood of patients with idiopathic bronchiectasis who were free of exacerbation, and from healthy controls of similar age (n=21 in both groups). Ingestion of serum-opsonised zymosan by neutrophils was used to quantify phagocytic capacity. Superoxide generation in neutrophils was measured in response to addition of platelet activating factor (PAF) and formyl-methionyl-leucyl-phenylalanine (fMLP). Experiments were performed in the presence or absence of GM-CSF.RESULTS: No differences were observed in either phagocytic capacity (p=0.99) or superoxide generation (p=0.81) when comparing patients and controls. However, GM-CSF induced a significant increase in phagocytic capacity above baseline levels in both patients (p<0.005) and controls (p<0.005). Similarly, GM-CSF significantly increased superoxide generation in patients (p<0.005) and controls (p=0.001).CONCLUSIONS: Peripheral blood neutrophil function was preserved in idiopathic bronchiectasis. Enhancement of neutrophil phagocytosis and superoxide generation by GM-CSF requires further study
Author(s): Ruchaud-Sparagano MH, Gertig H, Hester KL, Macfarlane JG, Corris P, Simpson AJ, De Soyza A
Publication type: Article
Publication status: Published
Journal: Respirology
Year: 2013
Volume: 18
Issue: 8
Pages: 1230-1235
Print publication date: 28/10/2013
ISSN (print): 1323-7799
ISSN (electronic): 1440-1843
Publisher: Wiley-Blackwell
URL: http://dx.doi.org/10.1111/resp.12138
DOI: 10.1111/resp.12138
PubMed id: 23730995
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