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Brief Report: A Human Induced Pluripotent Stem Cell Model of Cernunnos Deficiency Reveals an Important Role for XLF in the Survival of the Primitive Hematopoietic Progenitors

Lookup NU author(s): Dr Katarzyna Tilgner, Dr Irina Neganova, Chatcha Singhapol, Dr Gabriele Saretzki, Jerome Evans, Professor Andrew GenneryORCiD, Professor Miodrag Stojkovic, Professor Lyle Armstrong, Professor Majlinda LakoORCiD


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Cernunnos (also known as XLF) deficiency syndrome is a rare recessive autosomal disorder caused by mutations in the XLF gene, a key factor involved in the end joining step of DNA during nonhomologous end joining (NHEJ) process. Human patients with XLF mutations display microcephaly, developmental and growth delays, and severe immunodeficiency. While the clinical phenotype of DNA damage disorders, including XLF Syndrome, has been described extensively, the underlying mechanisms of disease onset, are as yet, undefined. We have been able to generate an induced pluripotent stem cell (iPSC) model of XLF deficiency, which accurately replicates the double-strand break repair deficiency observed in XLF patients. XLF patient-specific iPSCs (XLF-iPSC) show typical expression of pluripotency markers, but have altered in vitro differentiation capacity and an inability to generate teratomas comprised of all three germ layers in vivo. Our results demonstrate that XLF-iPSCs possess a weak NHEJ-mediated DNA repair capacity that is incapable of coping with the DNA lesions introduced by physiological stress, normal metabolism, and ionizing radiation. XLF-iPSC lines are capable of hematopoietic differentiation; however, the more primitive subsets of hematopoietic progenitors display increased apoptosis in culture and an inability to repair DNA damage. Together, our findings highlight the importance of NHEJ-mediated-DNA repair in the maintenance of a pristine pool of hematopoietic progenitors during human embryonic development. Stem Cells2013;31:2015-2023

Publication metadata

Author(s): Tilgner K, Neganova I, Singhapol C, Saretzki G, Al-Aama JY, Evans J, Gorbunova V, Gennery A, Przyborski S, Stojkovic M, Armstrong L, Jeggo P, Lako M

Publication type: Article

Publication status: Published

Journal: Stem Cells

Year: 2013

Volume: 31

Issue: 9

Pages: 2015-2023

Print publication date: 01/09/2013

Online publication date: 04/10/2013

Acceptance date: 21/05/2013

ISSN (print): 1066-5099

ISSN (electronic): 1549-4918

Publisher: Wiley-Blackwell


DOI: 10.1002/stem.1456


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Funder referenceFunder name
Conselleria de Sanidad (Generalitat Valenciana)
Instituto de Salud Carlos III (Ministry of Science and Innovation), Spain.
09005Leukemia and Lymphoma Research