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Human Dermal CD14+ Cells Are a Transient Population of Monocyte-Derived Macrophages

Lookup NU author(s): Dr Naomi McGovern, Dr Merry Gunawan, Dr Laura Jardine, Dr Elizabeth Poyner, Kile Green, Dr Rachel Dickinson, Dr Xiao Wang, Katie Best, Dr Paul Milne, Sarah Pagan, Dr Venetia Bigley, Professor Matthew Collin, Professor Muzlifah Haniffa

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Dendritic cells (DCs), monocytes, and macrophages are leukocytes with critical roles in immunity and tolerance. The DC network is evolutionarily conserved; the homologs of human tissue CD141(hi) XCR1(+)CLEC9A(+) DCs and CD1c(+) DCs are murine CD103(+) DCs and CD64(-)CD11b(+) DCs. In addition, human tissues also contain CD14(+) cells, currently designated as DCs, with an as-yet unknown murine counterpart. Here we have demonstrated that human dermal CD14(+) cells are a tissue-resident population of monocyte-derived macrophages with a short half-life of <6 days. The decline and reconstitution kinetics of human blood CD14(+) monocytes and dermal CD14(+) cells in vivo supported their precursor-progeny relationship. The murine homologs of human dermal CD14(+) cells are CD11b(+) CD64(+) monocyte-derived macrophages. Human and mouse monocytes and macrophages were defined by highly conserved gene transcripts, which were distinct from DCs. The demonstration of monocyte-derived macrophages in the steady state in human tissue supports a conserved organization of human and mouse mononuclear phagocyte system.


Publication metadata

Author(s): McGovern N, Schlitzer A, Gunawan M, Jardine L, Shin A, Poyner E, Green K, Dickinson R, Wang XN, Low D, Best K, Covins S, Milne P, Pagan S, Aljefri K, Windebank M, Saavedra DM, Larbi A, Wasan PS, Duan K, Poidinger M, Bigley V, Ginhoux F, Collin M, Haniffa M

Publication type: Article

Publication status: Published

Journal: Immunity

Year: 2014

Volume: 41

Issue: 3

Pages: 465-477

Print publication date: 18/09/2014

Online publication date: 04/09/2014

Acceptance date: 14/08/2014

Date deposited: 11/12/2014

ISSN (print): 1074-7613

ISSN (electronic): 1097-4180

Publisher: Cell Press

URL: http://dx.doi.org/10.1016/j.immuni.2014.08.006

DOI: 10.1016/j.immuni.2014.08.006


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