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Lookup NU author(s): Dr Frida Ponthan,
Professor Olaf Heidenreich,
Dr Kelly Coffey
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© 2015 by John Wiley & Sons, Inc.This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.
Author(s): Ponthan F, Yusoff NM, Soria NM, Heidenreich O, Coffey K
Publication type: Article
Publication status: Published
Journal: Current Protocols in Molecular Biology
Online publication date: 01/07/2015
Acceptance date: 01/01/1900
ISSN (print): 1934-3639
ISSN (electronic): 1934-3647
Publisher: Blackwell Publishing Inc.
URL: http://doi.org/ 10.1002/0471142727.mb2602s111
PubMed id: 26131850
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