Browse by author
Lookup NU author(s): Dr Stephanie Meyer, Dr Phillip Probert, Anne Lakey, Andrew Axon, Dr Alistair Leitch, Emeritus Professor Faith Williams, Dr Paul Jowsey, Professor Peter Blain, Professor George Kass, Professor Matthew Wright
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2017 The Authors Tartrazine is a food colour that activates the transcriptional function of the human oestrogen receptor alpha in an in vitro cell model. Since oestrogens are cholestatic, we hypothesised tartrazine will cause periportal injury to the liver in vivo. To test this hypothesis, tartrazine was initially administered systemically to mice resulting in a periportal recruitment of inflammatory cells, increased serum alkaline phosphatase activity and mild periportal fibrosis. To determine whether an oestrogenic effect may be a key event in this response, tartrazine, sulphonated metabolites and a food additive contaminant were screened for their ability to interact with murine oestrogen receptors. In all cases, there were no interactions as agonists or antagonists and further, no oestrogenicity was observed with tartrazine in an in vivo uterine growth assay. To examine the relevance of the hepatic effects of tartrazine to its use as a food additive, tartrazine was orally administered to transgenic NF-κB-Luc mice. Pre- and concurrent oral treatment with alcohol was incorporated given its potential to promote gut permeability and hepatic inflammation. Tartrazine alone induced NF- κB activities in the colon and liver but there was no periportal recruitment of inflammatory cells or fibrosis. Tartrazine, its sulphonated metabolites and the contaminant inhibited sulphotransferase activities in murine hepatic S9 extracts. Given the role of sulfotransferases in bile acid excretion, the initiating event giving rise to periportal inflammation and subsequent hepatic pathology through systemic tartrazine exposure is therefore potentially associated an inhibition of bile acid sulphation and excretion and not on oestrogen receptor-mediated transcriptional function. However, these effects were restricted to systemic exposures to tartrazine and did not occur to any significant effect after oral exposure.
Author(s): Meyer SK, Probert PME, Lakey AF, Axon AR, Leitch AC, Williams FM, Jowsey PA, Blain PG, Kass GEN, Wright MC
Publication type: Article
Publication status: Published
Journal: Toxicology Letters
Year: 2017
Volume: 273
Pages: 55-68
Print publication date: 05/05/2017
Online publication date: 27/03/2017
Acceptance date: 24/03/2017
Date deposited: 05/05/2017
ISSN (print): 0378-4274
ISSN (electronic): 1879-3169
Publisher: Elsevier Ireland
URL: https://doi.org/10.1016/j.toxlet.2017.03.024
DOI: 10.1016/j.toxlet.2017.03.024
Altmetrics provided by Altmetric
