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Lookup NU author(s): Dr Gary ReynoldsORCiD, James Fletcher, Dr Laura JardineORCiD, David Dixon, Dr Emily StephensonORCiD, Dr David McDonald, Professor Andrew FilbyORCiD, Professor Muzlifah Haniffa
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Association for the Advancement of Science, 2017.
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Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.
Author(s): Villani A-C, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, Griesbeck M, Butler A, Zheng S, Lazo S, Jardine L, Dixon D, Stephenson E, Nilsson E, Grundberg I, McDonald D, Filby A, Li W, De Jager PL, Rozenblatt-Rosen O, Lane AA, Haniffa M, Regev A, Hacohen N
Publication type: Article
Publication status: Published
Journal: Science
Year: 2017
Volume: 356
Issue: 6335
Print publication date: 21/04/2017
Online publication date: 21/04/2017
Acceptance date: 07/03/2017
Date deposited: 07/07/2017
ISSN (print): 0036-8075
ISSN (electronic): 1095-9203
Publisher: American Association for the Advancement of Science
URL: https://doi.org/10.1126/science.aah4573
DOI: 10.1126/science.aah4573
PubMed id: 28428369
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