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Lookup NU author(s): Dr Ian Cowell,
Professor Caroline AustinORCiD
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© 2018, Springer Science+Business Media, LLC.Chromatin immunoprecipitation is a method to isolate a protein of interest coupled to DNA following cross-linking with formaldehyde and to quantify the relative abundance or occupancy of the protein at specific genomic loci. After immunoprecipitation of protein–DNA complexes protein–DNA cross-links are reversed and the DNA is extracted. Various methods exist to identify binding sites and determine relative occupancy of the protein of interest; these include quantitative PCR, probing microarrays or sequencing the isolated DNA (ChIP-seq). This chapter details the method of chromatin immunoprecipitation of TOP2 to the point of DNA extraction from the precipitated protein–DNA complexes.
Author(s): Smith KA, Cowell IG, Austin CA
Publication type: Book Chapter
Publication status: Published
Book Title: DNA Topoisomerases: Methods and Protocols
Online publication date: 25/11/2017
Acceptance date: 02/04/2016
Series Title: Methods in Molecular Biology
Publisher: Humana Press
Place Published: New York
Library holdings: Search Newcastle University Library for this item