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Topoisomerase II chromatin immunoprecipitation

Lookup NU author(s): Dr Ian CowellORCiD, Professor Caroline AustinORCiD


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© 2018, Springer Science+Business Media, LLC.Chromatin immunoprecipitation is a method to isolate a protein of interest coupled to DNA following cross-linking with formaldehyde and to quantify the relative abundance or occupancy of the protein at specific genomic loci. After immunoprecipitation of protein–DNA complexes protein–DNA cross-links are reversed and the DNA is extracted. Various methods exist to identify binding sites and determine relative occupancy of the protein of interest; these include quantitative PCR, probing microarrays or sequencing the isolated DNA (ChIP-seq). This chapter details the method of chromatin immunoprecipitation of TOP2 to the point of DNA extraction from the precipitated protein–DNA complexes.

Publication metadata

Author(s): Smith KA, Cowell IG, Austin CA

Publication type: Book Chapter

Publication status: Published

Book Title: DNA Topoisomerases: Methods and Protocols

Year: 2018

Volume: 1703

Pages: 183-189

Online publication date: 25/11/2017

Acceptance date: 02/04/2016

Series Title: Methods in Molecular Biology

Publisher: Humana Press

Place Published: New York


DOI: 10.1007/978-1-4939-7459-7_14

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493974597