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Lookup NU author(s): Dr Valeria Chichagova,
Dr Birthe HilgenORCiD,
Dr Maria Georgiou,
Professor Lyle Armstrong,
Professor Majlinda LakoORCiD
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© 2019 John Wiley & Sons, Inc.This unit describes a protocol for generating retinal organoids that contain all major retinal cell types and are responsive to light from human pluripotent stem cells (hPSCs). hPSCs are differentiated in 96-well plates to allow large-scale production of organoids that could be used for multiple applications, including study of human retinal development, disease modeling, and compound screening. The differentiation approach is based on the knowledge that insulin-like growth factor 1 signaling together with retinoic acid and triiodothyronine is important for retinal development. After 22 weeks in culture, the organoids form a thick layer of neuroepithelium containing photoreceptors and bipolar, horizontal, amacrine, Müller, and retinal ganglion cells. Differentiation progress can be tracked by morphological observations and protein localization, as detected with immunocytochemistry. © 2019 by John Wiley & Sons, Inc.
Author(s): Chichagova V, Dorgau B, Felemban M, Georgiou M, Armstrong L, Lako M
Publication type: Article
Publication status: Published
Journal: Current Protocols in Stem Cell Biology
Online publication date: 31/07/2019
Acceptance date: 02/04/2016
ISSN (print): 1941-7322
ISSN (electronic): 1938-8969
Publisher: Blackwell Publishing Inc.
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