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Lookup NU author(s): Jose Coelho Lima Junior, Dr Ashfaq Mohammed, Dr Suzanne Cormack, Adnan Ali, Dr Matthew Barter, Dr Alan Bagnall, Professor Simi Ali, Professor David YoungORCiD, Professor Ioakim SpyridopoulosORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Association for Clinical Chemistry, 2020.
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Background: Failed myocardial reperfusion occurs in up to 50% of patients with ST elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention (PPCI). It is strongly associated with worse clinical outcome but is not routinely screened for. Muscle-enriched microribonucleic acids (miRNAs) levels are deregulated following STEMI. Whether they inform about failed myocardial reperfusion is poorly understood. Our aim was to investigate the release kinetics of miRNAs following PPCI and their association with failed myocardial reperfusion in patient with STEMI. Methods and results: Two candidate miRNAs (miR-1 and miR-133b) were identified following screening of 2,083 miRNAs by next generation sequencing in plasma from STEMI patients with (n=6) and without (n=6) failed myocardial reperfusion or healthy controls (n = 4). Measurements of miR-1 and miR-113b were performed at 13 time points within 3h post-reperfusion in 20 STEMI patients using TaqMan small RNA assays by RT-qPCR. This revealed that miR-1/miR-133b are rapidly released following reperfusion in a monophasic or biphasic pattern. In a ‘validation’ STEMI cohort (n = 50), miR-1 and miR-133b levels at 90min post-PPCI were approximately 3-fold (p = 0.001) and 4.4-fold (p = 0.008) higher in patients with failed myocardial reperfusion as assessed by cardiac resonance cardiac (CMR) imaging, respectively. In addition, miR-1 was particularly elevated in a subgroup of patients with worse left ventricular (LV) functional recovery 3 months post-PPCI. Conclusion: miR-1 and miR-133b levels increase within 3h of PPCI. They are positively associated with failed myocardial reperfusion and worse LV functional recovery post-PPCI.
Author(s): Coelho-Lima J, Mohammed A, Cormack S, Jones S, Ali A, Panahi P, Barter M, Bagnall A, Ali S, Young D, Spyridopoulos I
Publication type: Article
Publication status: Published
Journal: Clinical Chemistry
Year: 2020
Volume: 66
Issue: 1
Pages: 247-256
Print publication date: 01/01/2020
Online publication date: 31/10/2019
Acceptance date: 17/09/2019
Date deposited: 04/10/2019
ISSN (print): 0009-9147
ISSN (electronic): 1530-8561
Publisher: American Association for Clinical Chemistry
URL: https://doi.org/10.1373/clinchem.2019.308353
DOI: 10.1373/clinchem.2019.308353
PubMed id: 31672851
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