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Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids

Lookup NU author(s): Dr Maria Georgiou, Dr Valeria Chichagova, Dr Birthe HilgenORCiD, Professor Evelyne SernagorORCiD, Professor Lyle Armstrong, Professor Majlinda LakoORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


The generation of laminated and light responsive retinal organoids from induced pluripotent stem cells (iPSCs) provides a powerful tool for the study of retinal diseases and drug discovery and a robust platform for cell-based therapies. The aim of this study is to investigate whether retinal organoids can retain their morphological and functional characteristics upon storage at room temperature (RT) conditions and shipment by air using a commercially available container that maintains the environment at ambient temperature. Morphological analysis and measurements of neuroepithelial thickness revealed no differences between control, RT incubated and shipped organoids. Similarly immunohistochemical analysis showed no differences in cell type composition and position within the laminated retinal structure. All groups showed a similar response to light, suggesting that the biological function of retinal organoids was not affected by RT storage or shipment. These findings provide an advance in transport of ready-made retinal organoids, increasing their availability to many research and pharma labs worldwide and facilitating cross-collaborative research.

Publication metadata

Author(s): Georgiou M, Chichagova V, Hilgen G, Dorgau B, Sernagor E, Armstrong L, Lako M

Publication type: Article

Publication status: Published

Journal: PlosOne

Year: 2020

Volume: 15

Issue: 6

Online publication date: 01/06/2020

Acceptance date: 13/05/2020

Date deposited: 10/06/2020

ISSN (electronic): 1932-6203

Publisher: Public Library of Science


DOI: 10.1371/journal.pone.0233860


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