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Lookup NU author(s): Dr Maria Georgiou, Dr Valeria Chichagova, Dr Birthe HilgenORCiD, Professor Evelyne SernagorORCiD, Professor Lyle Armstrong, Professor Majlinda LakoORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The generation of laminated and light responsive retinal organoids from induced pluripotent stem cells (iPSCs) provides a powerful tool for the study of retinal diseases and drug discovery and a robust platform for cell-based therapies. The aim of this study is to investigate whether retinal organoids can retain their morphological and functional characteristics upon storage at room temperature (RT) conditions and shipment by air using a commercially available container that maintains the environment at ambient temperature. Morphological analysis and measurements of neuroepithelial thickness revealed no differences between control, RT incubated and shipped organoids. Similarly immunohistochemical analysis showed no differences in cell type composition and position within the laminated retinal structure. All groups showed a similar response to light, suggesting that the biological function of retinal organoids was not affected by RT storage or shipment. These findings provide an advance in transport of ready-made retinal organoids, increasing their availability to many research and pharma labs worldwide and facilitating cross-collaborative research.
Author(s): Georgiou M, Chichagova V, Hilgen G, Dorgau B, Sernagor E, Armstrong L, Lako M
Publication type: Article
Publication status: Published
Journal: PlosOne
Year: 2020
Volume: 15
Issue: 6
Online publication date: 01/06/2020
Acceptance date: 13/05/2020
Date deposited: 10/06/2020
ISSN (electronic): 1932-6203
Publisher: Public Library of Science
URL: https://doi.org/10.1371/journal.pone.0233860
DOI: 10.1371/journal.pone.0233860
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