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Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta

Lookup NU author(s): Mushtaq Khazeem, Dr Ian CowellORCiD, Lauren Harkin, John CasementORCiD, Professor Caroline AustinORCiD

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Abstract

© 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical SocietiesDNA topoisomerase II beta (TOP2B) has a role in transcriptional regulation. Here, to further investigate transcriptional regulation by TOP2B, we used RNA-sequencing and real-time PCR to analyse the differential gene expression profiles of wild-type and two independent TOP2B-null pre-B Nalm-6 cell lines, one generated by targeted insertion and the other using CRISPR-Cas9 gene editing. We identified carbonyl reductase 1 (CBR1) among the most significantly downregulated genes in these TOP2B-null cells. Reduced CBR1 expression was accompanied by loss of binding of the transcription factors USF2 and MAX to the CBR1 promoter. We describe possible mechanisms by which loss of TOP2B results in CBR1 downregulation. To our knowledge, this is the first report of a link between TOP2B and CBR1.

Publication metadata

Author(s): Khazeem MM, Cowell IG, Harkin LF, Casement JW, Austin CA

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2020

Volume: 594

Issue: 20

Pages: 3395-3405

Online publication date: 06/08/2020

Acceptance date: 31/07/2020

Date deposited: 11/12/2020

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Wiley Blackwell

URL: https://doi.org/10.1002/1873-3468.13904

DOI: 10.1002/1873-3468.13904

PubMed id: 32767399

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