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Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson’s disease

Lookup NU author(s): Dr Chun Chen, Dr David McDonald, Dr Alasdair Blain, Dr Ashwin Sachdeva, Laura Bone, Anna Smith, Charlotte Warren, Dr Sarah Pickett, Dr Gavin Hudson, Dr Andrew Filby, Dr Amy Vincent, Emeritus Professor Doug Turnbull, Dr Amy Reeve

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2021, The Author(s).Here we report the application of a mass spectrometry-based technology, imaging mass cytometry, to perform in-depth proteomic profiling of mitochondrial complexes in single neurons, using metal-conjugated antibodies to label post-mortem human midbrain sections. Mitochondrial dysfunction, particularly deficiency in complex I has previously been associated with the degeneration of dopaminergic neurons in Parkinson’s disease. To further our understanding of the nature of this dysfunction, and to identify Parkinson’s disease specific changes, we validated a panel of antibodies targeting subunits of all five mitochondrial oxidative phosphorylation complexes in dopaminergic neurons from Parkinson’s disease, mitochondrial disease, and control cases. Detailed analysis of the expression profile of these proteins, highlighted heterogeneity between individuals. There is a widespread decrease in expression of all complexes in Parkinson’s neurons, although more severe in mitochondrial disease neurons, however, the combination of affected complexes varies between the two groups. We also provide evidence of a potential neuronal response to mitochondrial dysfunction through a compensatory increase in mitochondrial mass. This study highlights the use of imaging mass cytometry in the assessment and analysis of expression of oxidative phosphorylation proteins, revealing the complexity of deficiencies of these proteins within individual neurons which may contribute to and drive neurodegeneration in Parkinson’s disease.


Publication metadata

Author(s): Chen C, McDonald D, Blain A, Sachdeva A, Bone L, Smith ALM, Warren C, Pickett SJ, Hudson G, Filby A, Vincent AE, Turnbull DM, Reeve AK

Publication type: Article

Publication status: Published

Journal: npj Parkinson's Disease

Year: 2021

Volume: 7

Issue: 1

Online publication date: 12/05/2021

Acceptance date: 29/03/2021

Date deposited: 18/06/2021

ISSN (electronic): 2373-8057

Publisher: Nature Research

URL: https://doi.org/10.1038/s41531-021-00182-x

DOI: 10.1038/s41531-021-00182-x


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