Toggle Main Menu Toggle Search

Open Access padlockePrints

A human model of bilateral pulmonary vein sampling to assess the effects of one-lung ventilation on neutrophil function

Lookup NU author(s): Dr Wendy Funston, Dr Marie-Helene Ruchaud, Jonathan Scott, Jason PowellORCiD, Dr Faye Cooles, Lauren Shelmerdine, Karen Booth, Stephen Clark, Dr Anthony RostronORCiD, Professor John Dark, Professor John SimpsonORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2022 Funston et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Background Neutrophil activation drives lung complications after cardiopulmonary bypass (CPB). Evidence suggests the healthy, ventilated lung may beneficially re-condition pro-inflammatory neutrophils. However, evidence in humans is lacking, due to a paucity of good models. CPB with simultaneous central venous and bilateral pulmonary vein sampling provides an opportunity to model effects of one-lung ventilation. The study's primary objectives were to establish a model of intra-operative, bilateral pulmonary vein sampling and to determine whether neutrophil function differed after passing through inflated or deflated lungs. Methods Seventeen patients having "on pump"coronary artery bypass grafting (CABG) with onelung ventilation (in two cohorts with tidal volume 2ml kg-1 and FiO2 0.21, or tidal volume 4 ml kg-1 and FiO2 0.5 respectively) were recruited. Cohort 1 consisted of 9 patients (7 male, median age 62.0 years) and Cohort 2 consisted of 8 male patients (median age 65.5 years). Recruitment was via prospective screening of scheduled elective and non-elective CABG procedures with cardiopulmonary bypass. Each patient had five blood samples taken - central venous blood pre-operatively; central venous blood pre-CPB; central venous blood post-CPB; pulmonary venous blood draining the ventilated lung post-CPB; and pulmonary venous blood draining the deflated lung post-CPB. Neutrophil phagocytosis and priming status were quantified. Plasma cytokines were measured. Results Phagocytosis and priming were not significantly different in neutrophils returning from the ventilated lung as compared to the non-ventilated lung. Plasma IL-6, IL-8 and IL-10 were significantly elevated by CPB. Conclusions The intra-operative, bilateral pulmonary vein sampling model provides unique opportunities to assess biological effects of interventions to one lung, with the other lung acting as an internal control. Single-lung ventilation during CPB had no significant effects on neutrophil function. Copyright:

Publication metadata

Author(s): Funston W, Ruchaud-Sparagano M-H, Scott J, Powell J, Cooles FAH, Shelmerdine L, McDowell C, O'Leary D, Booth KL, Clark SC, Ledingham SJ, Rostron AJ, Dark JH, Simpson AJ

Publication type: Article

Publication status: Published

Journal: PLoS ONE

Year: 2022

Volume: 17

Issue: 7

Online publication date: 26/07/2022

Acceptance date: 11/07/2022

Date deposited: 08/08/2022

ISSN (electronic): 1932-6203

Publisher: Public Library of Science


DOI: 10.1371/journal.pone.0271958


Altmetrics provided by Altmetric


Funder referenceFunder name
National Institute for Health Research (NIHR) Newcastle Biomedical Research Centre awarded to AJS, reference number BH142282 / PD0131