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Lookup NU author(s): Dr Annachiara ScalzoneORCiD, Dr Xiao WangORCiD, Dr Ana Ferreira-DuarteORCiD, Professor Kenneth Dalgarno, Dr Piergiorgio GentileORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.Osteoarthritis (OA) is a joint degenerative pathology characterized by mechanical and inflammatory damages affecting synovium, articular cartilage (AC), and subchondral bone (SB). Several in vitro, in vivo, and ex vivo models are developed to study OA, but to date the identification of specific pharmacological targets seems to be hindered by the lack of models with predictive capabilities. This study reports the development of a biomimetic in vitro model of AC and SB interface. Gellan gum methacrylated and chondroitin sulfate/dopamine hydrogels are used for the AC portion, whereas polylactic acid functionalized with gelatin and nanohydroxyapatite for the SB. The physiological behavior of immortalized stem cells (Y201s) and Y201s differentiated in chondrocytes (Y201-Cs), respectively, for the SB and AC, is demonstrated over 21 days of culture in vitro in healthy and pathological conditions, whilst modeling the onset of cytokines-induced OA. The key metrics are: lower glycosaminoglycans production and increased calcification given by a higher Collagen X content, in the AC deep layer; higher expression of pro-angiogenic factor (vegf) and decreased expression of osteogenic markers (coll1, spp1, runx2) in the SB. This novel approach provides a new tool for studying the development and progression of OA.
Author(s): Scalzone A, Cerqueni G, Wang X-N, Ferreira-Duarte A, Dalgarno K, Mattioli-Belmonte M, Gentile P
Publication type: Article
Publication status: Published
Journal: Advanced Healthcare Materials
Year: 2022
Volume: 12
Issue: 2
Pages: 2202030
Print publication date: 13/01/2023
Online publication date: 27/10/2022
Acceptance date: 27/09/2022
Date deposited: 22/11/2022
ISSN (print): 2192-2640
ISSN (electronic): 2192-2659
Publisher: John Wiley and Sons Inc
URL: https://doi.org/10.1002/adhm.202202030
DOI: 10.1002/adhm.202202030
PubMed id: 36300892
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