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The RNA editor ADAR2 promotes immune cell trafficking by enhancing endothelial responses to interleukin-6 during sterile inflammation

Lookup NU author(s): Dr Aikaterini GatsiouORCiD, Dr Simon Tual-Chalot, Dr Georgios Georgiopoulos, Professor Kimon Stamatelopoulos, Professor Ioakim SpyridopoulosORCiD, Professor Konstantinos StellosORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2023 The AuthorsImmune cell trafficking constitutes a fundamental component of immunological response to tissue injury, but the contribution of intrinsic RNA nucleotide modifications to this response remains elusive. We report that RNA editor ADAR2 exerts a tissue- and stress-specific regulation of endothelial responses to interleukin-6 (IL-6), which tightly controls leukocyte trafficking in IL-6-inflamed and ischemic tissues. Genetic ablation of ADAR2 from vascular endothelial cells diminished myeloid cell rolling and adhesion on vascular walls and reduced immune cell infiltration within ischemic tissues. ADAR2 was required in the endothelium for the expression of the IL-6 receptor subunit, IL-6 signal transducer (IL6ST; gp130), and subsequently, for IL-6 trans-signaling responses. ADAR2-induced adenosine-to-inosine RNA editing suppressed the Drosha-dependent primary microRNA processing, thereby overwriting the default endothelial transcriptional program to safeguard gp130 expression. This work demonstrates a role for ADAR2 epitranscriptional activity as a checkpoint in IL-6 trans-signaling and immune cell trafficking to sites of tissue injury.


Publication metadata

Author(s): Gatsiou A, Tual-Chalot S, Napoli M, Ortega-Gomez A, Regen T, Badolia R, Cesarini V, Garcia-Gonzalez C, Chevre R, Ciliberti G, Silvestre-Roig C, Martini M, Hoffmann J, Hamouche R, Visker JR, Diakos N, Wietelmann A, Silvestris DA, Georgiopoulos G, Moshfegh A, Schneider A, Chen W, Guenther S, Backs J, Kwak S, Selzman CH, Stamatelopoulos K, Rose-John S, Trautwein C, Spyridopoulos I, Braun T, Waisman A, Gallo A, Drakos SG, Dimmeler S, Sperandio M, Soehnlein O, Stellos K

Publication type: Article

Publication status: Published

Journal: Immunity

Year: 2023

Volume: 56

Issue: 5

Pages: 979-997.e11

Print publication date: 09/05/2023

Online publication date: 25/04/2023

Acceptance date: 30/03/2023

Date deposited: 16/05/2023

ISSN (print): 1074-7613

ISSN (electronic): 1097-4180

Publisher: Cell Press

URL: https://doi.org/10.1016/j.immuni.2023.03.021

DOI: 10.1016/j.immuni.2023.03.021

PubMed id: 37100060


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Funding

Funder referenceFunder name
16SFRN29020000
22080
75732319
759248
394046768
490846870
CRC1382/403224013
HL135121-01
HL132067-01A1
I01 CX002291
SFB1123 TP A6/B5
SFB914/B01
SFB914/B08
TRR332/C02
SFB TR285/10-2
SFB/TRR 355/1
TRR332

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