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Lookup NU author(s): Patricia Pinol Jurado, Jose Verdú-DíazORCiD, Esther Fernández-SimónORCiD, Dr Conor LawlessORCiD, Dr Amy VincentORCiD, Alejandro Gonzalez Chamorro, Dr Elisa Villalobos VillegasORCiD, Dr Alexandra Monceau, Zoe Laidler, Priyanka Mehra, Dr James Clark, Professor Andrew FilbyORCiD, Dr David McDonald, Andrew Bowey, Professor Giorgio TascaORCiD, Professor Chiara Marini Bettolo, Professor Michela GuglieriORCiD, Professor Volker StraubORCiD, Professor Jordi Diaz ManeraORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024. The Author(s). Becker muscular dystrophy (BMD) is characterised by fiber loss and expansion of fibrotic and adipose tissue. Several cells interact locally in what is known as the degenerative niche. We analysed muscle biopsies of controls and BMD patients at early, moderate and advanced stages of progression using Hyperion imaging mass cytometry (IMC) by labelling single sections with 17 markers identifying different components of the muscle. We developed a software for analysing IMC images and studied changes in the muscle composition and spatial correlations between markers across disease progression. We found a strong correlation between collagen-I and the area of stroma, collagen-VI, adipose tissue, and M2-macrophages number. There was a negative correlation between the area of collagen-I and the number of satellite cells (SCs), fibres and blood vessels. The comparison between fibrotic and non-fibrotic areas allowed to study the disease process in detail. We found structural differences among non-fibrotic areas from control and patients, being these latter characterized by increase in CTGF and in M2-macrophages and decrease in fibers and blood vessels. IMC enables to study of changes in tissue structure along disease progression, spatio-temporal correlations and opening the door to better understand new potential pathogenic pathways in human samples.
Author(s): Pinol-Jurado P, Verdu-Diaz J, Fernandez-Simon E, Dominguez-Gonzalez C, Hernandez-Lain A, Lawless C, Vincent A, Gonzalez-Chamorro A, Villalobos E, Monceau A, Laidler Z, Mehra P, Clark J, Filby A, McDonald D, Rushton P, Bowey A, Alonso Perez J, Tasca G, Marini-Bettolo C, Guglieri M, Straub V, Suarez-Calvet X, Diaz-Manera J
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Year: 2024
Volume: 14
Issue: 1
Online publication date: 09/02/2024
Acceptance date: 11/01/2024
Date deposited: 26/02/2024
ISSN (electronic): 2045-2322
Publisher: Springer Nature
URL: https://doi.org/10.1038/s41598-024-51906-x
DOI: 10.1038/s41598-024-51906-x
Data Access Statement: The raw data of the analysis obtained as well as the images acquired are available for further research upon reasonable request to the corresponding author. Hypo, the software designed for the analysis of IMC images, is available for use for free through collaboration agreements between researchers and the corresponding author.
PubMed id: 38336890
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