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Enhanced expression and activity of DNA polymerase β in human ovarian tumor cells: impact on sensitivity towards antitumor agents

Lookup NU author(s): Dr Linda Hogarth, Lynne Minto

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Abstract

DNA polymerase β, one of the most inaccurate DNA synthesizing enzymes, has been shown to confer genetic instability when up-regulated in cells, a situation found in several human cancers. Here, we demonstrated that enhanced activity and expression of this enzyme occur in the human ovarian tumor 2008/C13*5.25 cells, which are resistant to the antitumor agent cisplatin and hypersensitive to 6-thioguanine. We found that translesion synthesis across platinated DNA crosslinks as well as increased incorporation into DNA of 6-thioguanine took place in the 2008/C13*5.25 cells compared to the parental 2008 cells. Such features being molecular signatures of DNA polymerase β, these findings suggest that deregulation of its expression in cancer cells may contribute to the modulation of the response to antitumor treatments and therefore to tumor progression.

Publication metadata

Author(s): Bergoglio V, Canitrot Y, Hogarth LA, Minto CLJ, Howell SB, Cazaux C, Hoffmann JS

Publication type: Article

Publication status: Published

Journal: Oncogene

Year: 2001

Volume: 20

Issue: 43

Pages: 6181-6187

Print publication date: 27/09/2001

ISSN (print): 0950-9232

ISSN (electronic): 1476-5594

URL: http://dx.doi.org/10.1038/sj.onc.1204743

DOI: 10.1038/sj.onc.1204743

PubMed id: 11593426

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