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Development of a two-site ELISA assay for the dimeric form of human TFF1

Lookup NU author(s): Dr Meiyi Pu, Dr Felicity May, Dr Marie-Helene Ruchaud, Professor Bruce Westley

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Abstract

TFF1 is one of three human trefoil proteins expressed principally in the gastrointestinal tract in normal tissues. TFF1 protects the gastric mucosa against damage as a result of its ability to facilitate reconstitution of damaged gastric mucosa and its involvement in the secretion and structure of gastric mucus. The most biologically active molecular form in cell culture and animal models tested is a dimer formed by a disulfide bond between two cysteine residues close to the C terminus of the protein. We have therefore developed an assay for this form of TFF1 which should facilitate its measurement in biological samples. © 2004 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Pu M, May FEB, Playford R, Ruchaud-Sparagano M, Westley BR

Publication type: Article

Publication status: Published

Journal: Peptides

Year: 2004

Volume: 25

Issue: 5

Pages: 731-736

Print publication date: 01/05/2004

ISSN (print): 0196-9781

ISSN (electronic): 1873-5169

URL: http://dx.doi.org/10.1016/j.peptides.2004.01.014

DOI: 10.1016/j.peptides.2004.01.014

PubMed id: 15177866

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