Toggle Main Menu Toggle Search

Open Access padlockePrints

Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages

Lookup NU author(s): Dr Louise Hyslop, Professor Miodrag Stojkovic, Professor Lyle Armstrong, Theresia Walter, Petra Stojkovic, Professor Mary Herbert, Professor Alison Murdoch, Professor Tom Strachan, Professor Majlinda LakoORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


The homeobox transcription factor Nanog has been proposed to play a crucial role in the maintenance of the undifferentiated state of murine embryonic stem cells. A human counterpart, NANOG, has been identified, but its function and localization have not hitherto been described. We have used a combination of RNA interference and quantitative real-time polymerase chain reaction to study NANOG in human embryonic stem and embryonic carcinoma cells. Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and ACG-beta. Immunostaining of preimplantation human embryos showed that NANOG was expressed in the inner cell mass of expanded blastocysts but not in earlier-stage embryos, consistent with a role in the maintenance of pluripotency. Taken together, our findings suggest that NANOG acts as a gate-keeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages. © AlphaMed Press.

Publication metadata

Author(s): Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M

Publication type: Article

Publication status: Published

Journal: Stem Cells

Year: 2005

Volume: 23

Issue: 8

Pages: 1035-1043

Print publication date: 01/09/2005

ISSN (print): 1066-5099

ISSN (electronic): 1549-4918

Publisher: AlphaMed Press, Inc.


DOI: 10.1634/stemcells.2005-0080

PubMed id: 15983365


Altmetrics provided by Altmetric


Funder referenceFunder name
G0301182Medical Research Council