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Lookup NU author(s): Dr Catherine ArdenORCiD,
Professor Loranne Agius
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The association of glucokinase with liver mitochondria has been reported [Danial et al. (2003) BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424, 952-956]. We confirmed association of glucokinase immunoreactivity with rat liver mitochondria using Percoll gradient centrifugation and demonstrated its association with the 68 kDa regulatory protein (GKRP) but not with the binding protein phosphofructokinase- 2/fructose bisphosphatase-2. Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP. Combined with previous observations that GKRP overexpression partially inhibits glycolysis [de la Iglesia et al. (2000) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the hepatocyte. J. Biol. Chem. 275, 10597-10603] these findings suggest that there may be distinct glycolytic pools of glucokinase. © 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Author(s): Arden C, Baltrusch S, Agius L
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Print publication date: 03/04/2006
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
PubMed id: 16542652
Notes: Edited by Vladimir Skulachev
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