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Lookup NU author(s): Dr Aiste McCormick, Gavin Cuthbert, Dr Rachel O'DonnellORCiD, Dr Brian Wilson, Huw ThomasORCiD, Dr Helen Blair, Dr Sarah FordhamORCiD, Professor John LunecORCiD, Professor James Allan, Professor Richard Edmondson
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Background: Cell lines provide a powerful model to study cancer and here we describe a new spontaneously immortalised epithelial ovarian cancer cell line (NUOC-1) derived from the ascites collected at a time of primary debulking surgery for a mixed endometrioid / clear cell / High Grade Serous (HGS) histology. Results: This spontaneously immortalised cell line was found to maintain morphology and epithelial markers throughout long-term culture. NUOC-1 cells grow as an adherent monolayer with a doubling time of 58 hours. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype. SNP array analysis of parent and derived subpopulations (NUOC-1-A1 and NUOC-1-A2) cells demonstrated heterogeneous cell populations with numerous copy number alterations and a pro-amplification phenotype. The characteristics of this new cell line lends it to be an excellent model for investigation of a number of the identified targets. Materials and Methods: The cell line has been characterised for growth, drug sensitivity, expression of common ovarian markers and mutations, clonogenic potential and ability to form xenografts in SCID mice. Copy number changes and clonal evolution were assessed by SNP arrays.
Author(s): McCormick A, Earp E, Elliot K, Cuthbert G, O'Donnell R, Wilson BT, Sutton R, Leeson C, Thomas HD, Blair H, Fordham S, Lunec J, Allan J, Edmondson RJ
Publication type: Article
Publication status: Published
Journal: Oncotarget
Year: 2017
Volume: 8
Issue: 16
Pages: 26832-26844
Online publication date: 01/03/2017
Acceptance date: 20/02/2017
Date deposited: 03/05/2017
ISSN (electronic): 1949-2553
Publisher: Impact Journals LLC
URL: https://doi.org/10.18632/oncotarget.15821
DOI: 10.18632/oncotarget.15821
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