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Lookup NU author(s): Dr Tarek Mamdouh AbdelghanyORCiD, Dr Alistair Leitch, Dr Ibrahim Ibrahim, Dr Satomi Miwa, Professor Colin Wilson, Professor Matt Wright
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Recent studies have identified the 8C alkyl chain methylimidazolium ionic liquid 1-octyl-3-methylimidazolium in the environment and its potential to trigger the auto-immune liver disease primary biliary cholangitis. The toxicity of a range of methylimidazolium ionic liquids were therefore examined. Oxygen consumption was rapidly inhibited, with potency increasing with alkyl chain length. This preceded caspase 3/7 induction and DNA fragmentation. Time- and dose-dependent loss of dye reduction capacities reflected these effects, with a >700 fold difference in potency between 2C and 10C alkyl chain liquids. None of the ionic liquids directly inhibited mitochondrial complexes I-IV or complex V (F0F1-ATPase). However, dithionite reduction and ESR spectroscopy studies indicate a one electron reduction of oxygen in the presence of a methylimidazolium ionic liquid, suggesting methylimidazolium ionic liquids function as mitochondrial electron acceptors. However, only longer chain ionic liquids form a non-aqueous phase or micelle under aqueous physiological conditions and lead to increases in reactive oxygen species in intact cells. These data therefore suggest that the longer chain methylimidazolium liquids are toxic in sensitive liver progenitor cells because they both readily integrate within the inner mitochondrial membrane and accept electrons from the electron chain, leading to oxidative stress.
Author(s): Abdelghany TM, Leitch AC, Nevjestic I, Ibrahim I, Miwa S, Wilson C, Heutz S, Wright MC
Publication type: Article
Publication status: Published
Journal: Food and Chemical Toxicology
Year: 2020
Volume: 145
Pages: 111593
Print publication date: 01/11/2020
Online publication date: 07/08/2020
Acceptance date: 08/07/2020
Date deposited: 19/08/2020
ISSN (print): 0278-6915
Publisher: Elsevier
URL: https://doi.org/10.1016/j.fct.2020.111593
DOI: 10.1016/j.fct.2020.111593
PubMed id: 32777338
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