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Lookup NU author(s): Dr Joseph Collin, Dr Agata Rozanska, Dr Veronika Boczonadi, Marina Moya Molina, Adrienne Unsworth, Raf Hussain, Dr Jonathan Coxhead, Dr Tamilvendhan Dhanaseelan, Professor Lyle Armstrong, Dr Rachel Queen, Professor Majlinda LakoORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Molecular information on the early stages of human retinal development remains scarce due to limitations in obtaining early human eye samples. Pluripotent stem cell-derived retinal organoids provide an unprecedented opportunity for studying early retinogenesis. Using a combination of single cell RNA-Seq and spatial transcriptomics we present for the first-time a single cell spatio-temporal transcriptome of retinal organoid development. Our data demonstrate that retinal organoids recapitulate key events of retinogenesis including optic vesicle/cup formation, presence of a putative ciliary margin zone, emergence of retinal progenitor cells and their orderly differentiation to retinal neurons. Combining the scRNA- with scATAC-Seq data, we were able to reveal cell-type specific transcription factor binding motifs on accessible chromatin at each stage of organoid development, and to show that chromatin accessibility is highly correlated to the developing human retina, but with some differences in the temporal emergence and abundance of some of the retinal neurons.
Author(s): Dorgau B, Collin J, Rozanska A, boczonadi V, Molina MM, Unsworth A, Hussain r, Coxhead J, Dhanaseelan T, Armstrong L, Queen R, Lako M
Publication type: Article
Publication status: Published
Journal: iScience
Year: 2024
Volume: 27
Issue: 4
Print publication date: 19/04/2024
Online publication date: 04/03/2024
Acceptance date: 28/02/2024
Date deposited: 29/08/2024
ISSN (electronic): 2589-0042
Publisher: Cell Press
URL: https://doi.org/10.1016/j.isci.2024.109397
DOI: 10.1016/j.isci.2024.109397
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