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FragLites - minimal, halogenated fragments displaying pharmacophore doublets. An efficient approach to druggability assessment and hit generation

Lookup NU author(s): Daniel Wood, Daniel Lopez Fernandez, Leanne Knight, Islam Al-Khawaldeh, Dr Conghao Gai, Dr Mathew Martin, Duncan MillerORCiD, Dr Celine CanoORCiD, Professor Jane Endicott, Dr Ian HardcastleORCiD, Professor Martin NobleORCiD, Professor Mike Waring



This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Chemical Society, 2019.

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Identifying ligand binding sites on proteins is a critical step in target-based drug discovery. Current approaches to this require resource intensive screening of large libraries of lead-like or fragment molecules. Here we describe an efficient and effective experimental approach to mapping interaction sites using a set of halogenated compounds expressing paired hydrogen-bonding motifs, termed FragLites. The FragLites identify productive drug-like interactions, which are identified sensitively and unambiguously by X-ray crystallography, exploiting the anomalous scattering of the halogen substituent. This mapping of protein interaction surfaces provides an assessment of druggability and can identify efficient start points for the de novo design of hit molecules incorporating the interacting motifs. The approach is illustrated by mapping cyclin-dependent kinase 2, which successfully identifies orthosteric and allosteric sites. The hits were rapidly elaborated to develop efficient lead-like molecules. Hence, the approach provides a new method of identifying ligand sites, assessing tractability and discovering new leads.

Publication metadata

Author(s): Wood D, Lopez-Fernandez JD, Knight LE, Al-Khawaldeh I, Gai C, Lin S, Martin MP, Miller DC, Cano C, Endicott JA, Hardcastle IR, Noble MEM, Waring MJ

Publication type: Article

Publication status: Published

Journal: Journal of Medicinal Chemistry

Year: 2019

Volume: 62

Issue: 7

Pages: 3741-3752

Print publication date: 11/04/2019

Online publication date: 12/03/2019

Acceptance date: 11/03/2019

Date deposited: 18/03/2019

ISSN (print): 0022-2623

ISSN (electronic): 1520-4804

Publisher: American Chemical Society


DOI: 10.1021/acs.jmedchem.9b00304

PubMed id: 30860382


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