Lookup NU author(s): Emily Dookun,
Dr Anna Walaszczyk,
Dr Rachael Redgrave,
Dr Simon Tual-Chalot,
Professor Ioakim Spyridopoulos,
Professor Helen Arthur,
Dr Joao Passos,
Dr Gavin Richardson
This is the authors' accepted manuscript of a conference proceedings (inc. abstract) that has been published in its final definitive form by Heart , 2018.
For re-use rights please refer to the publisher's terms and conditions.
Introduction Myocardial infarction (MI) is a prominent cause of morbidity and mortality globally. While reperfusion via primary percutaneous coronary intervention is the gold-standard therapy, it can lead to the phenomenon ischemia-reperfusion injury (IRI), characterised by progressive remodelling and heart failure. Little is understood regarding to the mechanisms driving IRI, however, increased oxidative stress is known to play a role. Our previous studies demonstrate that during ageing increased oxidative stress drives telomere associated DNA damage foci (TAF) induced cardiomyocyte senescence, which is directly associated with a hypertrophic phenotype. Furthermore senescent cardiomyocytes express a pro-fibrotic profile; in particular an up-regulation of TGF-β expression. We now hypothesise that cardiomyocyte senescence contributes to remodelling subsequent to MI and IRI via similar mechanisms, and as such represents a potential therapeutic target.Methods Young three month old mice underwent 60 min surgical ligation of the left anterior descending coronary artery to mimic MI followed by reperfusion. Histological analysis at numerous time points post-MI for several senescence markers was performed.Results and conclusions In support of our hypothesis, we have observed that within the surviving myocardium, proximal to the infarct region, cardiomyocytes acquire a senescent-like phenotype. This is demonstrated by an increase in senescence markers including SA-β-Gal, p21 and p16, in addition to an accumulation of TAF. As well as this we verified that mice displayed classical pathophysiological aspects related to MI. Using in vitro studies and transgenic mouse models we aim to better understand the biology underlying cardiomyocyte senescence, and to establish the mechanisms underlying senescence contribution to remodelling. Furthermore we aim to ascertain if modulation or clearance of cardiomyocyte senescence promotes regeneration and improves outcome following IRI.
Author(s): Dookun ER, Walaszczyk A, Redgrave R, Tual-Chalot S, Yausep O, Spyridopoulos I, Owens A, Arthur H, Passos J, Richardson GD
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: BAS/BSCR Spring Meeting 2018
Year of Conference: 2018
Print publication date: 01/06/2018
Online publication date: 01/06/2018
Acceptance date: 13/03/2018
Date deposited: 06/08/2018