Publications by Dr David Bolam - ePrints

Browsing publications by Dr David Bolam.

Newcastle Authors	Title	Year
Dr David Bolam Professor Frank Sargent Professor Tracy Palmer FRS FRSE FMedSci Dr Emmanuele Severi	Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria	2024
Dr David Bolam Professor Frank Sargent Professor Tracy Palmer FRS FRSE FMedSci Dr Emmanuele Severi	Corrigendum: Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria	2024
Dr David Bolam	Cryptic diversity of cellulose-degrading gut bacteria in industrialized humans	2024
Dr David Bolam	Discovery, structural characterization, and functional insights into a novel apiosidase from the GH140 family, isolated from a lignocellulolytic-enriched mangrove microbial community	2024
Dr Augustinas Silale Matthew Feasey Dr Tiaan Heunis Dr Yiling Zhu Dr Hong Zheng et al.	Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes	2023
Dr David Bolam	Diverse events have transferred genes for edible seaweed digestion from marine to human gut bacteria	2022
Dr Arnaud Basle Dr David Bolam	Plant N-glycan breakdown by human gut Bacteroides	2022
Declan Gray Amy Glenwright Dr Arnaud Basle Carl Morland Dr David Bolam et al.	Insights into SusCD-mediated glycan import by a prominent gut symbiont	2021
Dr David Bolam	A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG	2020
Dr Lucy Crouch Dr Arnaud Basle Professor Chris Lamb Professor Christopher Stewart Kate Cooke et al.	Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown	2020
Justina Briliūtė Dr Elisabeth Lowe Dr David Bolam Dr Lucy Crouch	Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci	2019
Dr Lucy Crouch Dr David Bolam	Cloning, purification and biochemical characterisation of a GH35 beta-1,3/beta-1,6-galactosidase from the mucin-degrading gut bacterium Akkermansia muciniphila	2018
Carl Morland Dr David Bolam	Genetic variation of the SusC/SusD homologs from a polysaccharide utilization locus underlies divergent fructan specificities and functional adaptation in Bacteroides thetaiotaomicron strains	2018
Dr David Bolam Professor Bert van den Berg	TonB-dependent transport by the gut microbiota: novel aspects of an old problem	2018
Dr Alan Cartmell Dr Elisabeth Lowe Dr Arnaud Basle Dr Susan Firbank Dr Didier Ndeh et al.	How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans	2017
Amy Glenwright Dr Arnaud Basle Dr Susan Firbank Dr Hong Zheng Dr David Bolam et al.	Structural basis for nutrient acquisition by dominant members of the human gut microbiota	2017
Dr Lucy Crouch Dr Fiona Cuskin Justina Briliūtė Dr Arnaud Basle Dr David Bolam et al.	Degradation of complex N-glycans by gut Bacteroides species	2016
Justina Briliūtė Dr Lucy Crouch Dr David Bolam	Insight into N-glycan breakdown by the gut microbiota	2016
Dr Fiona Cuskin Dr Lucy Crouch Dr Arnaud Basle Dr David Bolam Emeritus Professor Harry Gilbert et al.	Pivotal alpha mannosidase generates specificity for N-glycans through requirement for GlcNac at+2 subsite.	2016
Dr David Bolam Amy Glenwright	Structural basis for glycan acquisition by dominant members of the human gut microbiota	2016
Dr Artur Rogowski Jon Briggs Dr Elisabeth Lowe Dr Arnaud Basle Carl Morland et al.	Glycan complexity dictates microbial resource allocation in the large intestine	2015
Dr Artur Rogowski Dr Arnaud Basle Dr Alexandra Solovyova Emeritus Professor Harry Gilbert Dr David Bolam et al.	Evidence That GH115 alpha-Glucuronidase Activity, Which Is Required to Degrade Plant Biomass, Is Dependent on Conformational Flexibility	2014
Dr David Bolam	The pattern of xylan acetylation suggests xylan may interact with cellulose microfibrils as a twofold helical screw in the secondary plant cell wall of Arabidopsis thaliana	2014
Dr Elisabeth Lowe Dr David Bolam	Tuning transcription of nutrient utilization genes to catabolic rate promotes growth in a gut bacterium	2014
Dr Katalin Kovacs Dr Adam Jackson Dr David Bolam	Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824	2013
Dr Sirintra Nakjang Dr Didier Ndeh Professor Anil Wipat Dr David Bolam Professor Robert Hirt et al.	A Novel Extracellular Metallopeptidase Domain Shared by Animal Host-Associated Mutualistic and Pathogenic Microbes	2012
Dr Elisabeth Lowe Dr Arnaud Basle Dr Susan Firbank Dr David Bolam	A scissor blade-like closing mechanism implicated in transmembrane signaling in a Bacteroides hybrid two-component system	2012
Dr David Bolam	Glycan recognition by the Bacteroidetes Sus-like systems	2012
Dr David Bolam Emeritus Professor Harry Gilbert	Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode	2012
Dr David Bolam	Mechanistic insight into polysaccharide use within the intestinal microbiota	2011
Dr Elisabeth Lowe Emeritus Professor Harry Gilbert Dr David Bolam	Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts	2011
Cedric Montanier Dr James Flint Dr David Bolam He Fang Xie Ziyuan Liu et al.	Circular Permutation Provides an Evolutionary Link between Two Families of Calcium-dependent Carbohydrate Binding Modules	2010
Dr Susan Firbank Dr David Bolam	Specificity of Polysaccharide Use in Intestinal Bacteroides Species Determines Diet-Induced Microbiota Alterations	2010
Emeritus Professor Harry Gilbert Dr David Bolam	Family 6 carbohydrate-binding modules display multiple beta 1,3-linked glucan-specific binding interfaces	2009
Benedita Pinheiro Emeritus Professor Harry Gilbert Dr David Bolam	Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum	2009
Cedric Montanier Dr James Flint Benedita Pinheiro Carl Morland Alan Cartmell et al.	The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions	2009
Dr David Bolam Dr Mark Proctor Emeritus Professor Harry Gilbert	The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity	2007
Dr James Flint Dr David Bolam Emeritus Professor Harry Gilbert	Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules	2006
Joanna Henshaw Dr David Bolam Emeritus Professor Harry Gilbert	Family 6 carbohydrate binding modules in β-agarases display exquisite selectivity for the non-reducing termini of agarose chains	2006
Dr Mark Proctor Dr David Bolam Emeritus Professor Harry Gilbert	Insights into the Synthesis of Lipopolysaccharide and Antibiotics through the Structures of Two Retaining Glycosyltransferases from Family GT4	2006
Dr James Flint Dr David Bolam Emeritus Professor Harry Gilbert	Understanding the biological rationale for the diversity of cellulose-directed carbohydrate-binding modules in prokaryotic enzymes	2006
Emeritus Professor Harry Gilbert Dr David Bolam	Xyloglucan is recognized by carbohydrate-binding modules that interact with β-glucan chains	2006
Dr Mark Proctor Dr David Bolam Emeritus Professor Harry Gilbert	Probing the breadth of macrolide glycosyltransferases: In vitro remodeling of a polyketide antibiotic creates active bacterial uptake and enhances potency	2005
Dr James Flint Dr David Bolam Dr Edward Taylor Emeritus Professor Harry Gilbert	Probing the mechanism of ligand recognition in family 29 carbohydrate-binding modules	2005
Dr James Flint Dr David Bolam Dr Louise Tailford Emeritus Professor Harry Gilbert	Structural dissection and high-throughput screening of mannosylglycerate synthase	2005
Dr David Bolam Gavin Pell Emeritus Professor Harry Gilbert	Structure of a mannan-specific family 35 carbohydrate-binding module: Evidence for significant conformational changes upon ligand binding	2005
Dr David Bolam Emeritus Professor Harry Gilbert	Carbohydrate-binding modules: Fine-tuning polysaccharide recognition	2004
Emeritus Professor Harry Gilbert Dr David Bolam	Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers	2004
Dr James Flint Emeritus Professor Harry Gilbert Dr David Bolam	Ligand-mediated dimerization of a carbohydrate-binding module reveals a novel mechanism for protein-carbohydrate recognition	2004
Dr David Bolam Emeritus Professor Harry Gilbert	Reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants	2004
Joanna Henshaw Dr David Bolam Emeritus Professor Harry Gilbert	The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5A in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities	2004
Dr David Bolam Emeritus Professor Harry Gilbert	The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates β-1,4- and β-1,3-1,4-mixed linked glucans at a single binding site	2004
Joanna Henshaw Dr David Bolam Emeritus Professor Harry Gilbert	The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities	2004
Dr David Bolam Gavin Pell Deborah Hogg Greta Galbraith Emeritus Professor Harry Gilbert et al.	X4 modules represent a new family of carbohydrate-binding modules that display novel properties	2004
Gavin Pell Huamao Du Emeritus Professor Harry Gilbert Dr David Bolam	Importance of hydrophobic and polar residues in ligand binding in the family 15 carbohydrate-binding module from Cellvibrio japonicus Xyn10C	2003
Tibor Nagy Professor Jeremy Lakey Dr David Bolam Emeritus Professor Harry Gilbert	The α-Glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants	2003
Emeritus Professor Harry Gilbert Dr David Bolam	Calcium binding and thermostability of carbohydrate binding module CBM4-2 of Xyn10A from Rhodothermus marinus	2002
Dr David Bolam Lorand Szabo Dr Vincent McKie Emeritus Professor Harry Gilbert	Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello-and mannohexaose	2002
Dr David Bolam Emeritus Professor Harry Gilbert	A Novel Carbohydrate-binding Protein Is a Component of the Plant Cell Wall-degrading Complex of Piromyces equi	2001
He Fang Xie Emeritus Professor Harry Gilbert Dr David Bolam	Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: The role of conserved amino acids in ligand binding	2001
Deborah Hogg Dr David Bolam Dr Vincent McKie Emeritus Professor Harry Gilbert	Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding	2001
Dr David Bolam Emeritus Professor Harry Gilbert	Evidence for synergy between family 2b carbohydrate binding modules in Cellulomonas fimi Xylanase 11A	2001
Lorand Szabo He Fang Xie Dr David Bolam Emeritus Professor Harry Gilbert	Structure of a Family 15 Carbohydrate-binding Module in Complex with Xylopentaose	2001
Lorand Szabo He Fang Xie Dr David Bolam Emeritus Professor Harry Gilbert	Structure of a family 15 carbohydrate-binding module in complex with xylopentaose: Evidence that xylan binds in an approximate 3-fold helical conformation	2001
Dr David Bolam Emeritus Professor Harry Gilbert	The Location of the Ligand-binding Site of Carbohydrate-binding Modules That Have Evolved from a Common Sequence Is Not Conserved	2001
He Fang Xie Dr David Bolam Emeritus Professor Harry Gilbert	The structural basis for the ligand specificity of family 2 carbohydrate-binding modules	2000
Dr David Bolam Emeritus Professor Harry Gilbert	The X6 'thermostabilizing' domains of xylanases are carbohydrate-binding modules: Structure and biochemistry of the Clostridium thermocellum X6b domain	2000
Dr David Bolam Emeritus Professor Harry Gilbert	A family IIb xylan-binding domain has a similar secondary structure to a homologous family IIa cellulose-binding domain but different ligand specificity	1999
Dr David Bolam Emeritus Professor Harry Gilbert	The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism	1999
Dr David Bolam Emeritus Professor Harry Gilbert	Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity	1998
Dr David Bolam Emeritus Professor Harry Gilbert	Cellulose binding domains and linker sequences potentiate the activity of hemicellulases against complex substrates	1997
Dr David Bolam Dr Richard Virden Professor Jeremy Lakey Emeritus Professor Harry Gilbert	Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues	1996